The tongue-in-cheek band, led by Dierks Bentley, announced their return earlier in May.
The Mississippi songsmith joins the likes of Lainey Wilson, Parker McCollum and Blake Shelton on the star-studded bill.
Also set to perform are the likes of Sam Hunt, Jordan Davis, Lady A and Ian Munsick.
The genre-fusing track appears on the Illinois rapper's new album, Almost Healed.
The special collaboration has been released as an exclusive Spotify Single.
Molly Tuttle, The Wood Brothers, Brent Cobb, Mike & The Moonpies and more have all been confirmed for the Florida festival.
