Ernest was performing as part of the ‘Losers Birthday Party’, which also featured Riley Green, Jamey Johnson and more.
The collaboration appears on Tuttle's celebrated 2023 album, ‘City of Gold’.
The ‘Black and Yellow’ hitmaker surprised fans during Wallen's encore.
Chesney accompanied the clip with a heartfelt tribute to the late ‘Margaritaville’ icon.
The sun-soaked anthem has enjoyed a resurgence in popularity following the country icon's death on September 1 2023.
The self-titled project has debuted at the top of the prestigious all-genre chart.